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Bioindicators for the Assessment of Microbiological Purity in Some Alimentary Products

Research and development

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Abstract:

A method is presented for the preparation of fluorescence bioindicators in the form of a film. The base film is made of either cellulose acetate or cellulose acetate-covered paperboard prepared from a bio-modified cellulose. The film contains fluorescein acetate (FDA). It has been documented that the bioindicators reveal a colour change in UV light when in contact with esterases produced by microorganisms and accumulated in meat to an amount exceeding the permissible limits. The hue change toward yellow-green proceeds as a consequence of the liberation of free fluorescein by the action of enzymes from the esterase group. The released fluorescein remains in the bioindicator and does not migrate to the tested product. An intensity increase of the excited light (R+G) to the level 90 units indicates decay of the product.

Tags: bioindicator, fluorescein acetate (FDA), film, food freshness.

Published in issue no 6 (71) / 2008, pages 122–126.

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