Abstract:

In this study, starch-protein raw material was esterified with oleic acid, vinyl laurate as well as rape oil as an acyl donor. Chloroform was used as a reaction medium and  immobilized lipase from Candida antarctica as a catalyst. The degree of substitution (DS) of the products was determined by the volumetric method. The effect of the reaction parameters such as the kind of  acyl donor or scale of the process on the DS, was studied. The product with the highest DS (in small scale - 0.11) was found in the reaction carried out at 50 °C for 6 h with  oleic acid. Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses confirmed the esterification of the starch-protein material. The hydrophobicity of non-esterified material and the final product was evaluated using the sessile drop method according to European Pharmacopeia 8.0. The possibility of processing the product of esterification into pellets to be used for film and fittings production was examined.